Background: Allopurinol, a common medication for gout treatment, can cause rare but life-threatening severe cutaneous adverse reactions (SCARs). Strong pharmacogenetic association of HLA-B*58:01 with allopurinol-induced drug hypersensitivity has been reported especially in Han Chinese population. Objectives: To develop the rapid and simple Loop-Mediated Isothermal Amplification (LAMP) assay of HLA-B*58:01 and evaluate its feasibility in predicting allopurinol-induced drug hypersensitivity. Methods: Two sets of LAMP primers targeting exons 2 and 3 of HLA B*58:01 were designed. Extracted DNA from twenty clinical blood samples of gout patients were used to evaluate the effectiveness of the two LAMP primers sets for the detection of HLA B*58:01. Results: Results were compared with routine clinical genotyping methods. All extracted DNA samples tested with the HLA-B*58:01 LAMP assay showed agreement with the routine genotyping results. No amplifications were observed when unextracted blood samples were tested. Conclusions: The HLA-B*58:01 LAMP assay was confirmed to be simple, rapid and specific for the detection of HLA-B*58:01 and therefore of potential value in the diagnosis of allopurinol-induced hypersensitivity.
Background: Allopurinol, a common medication for gout treatment, can cause rare but life-threatening severe cutaneous adverse reactions (SCARs). Strong pharmacogenetic association of HLA-B*58:01 with allopurinol-induced drug hypersensitivity has been reported especially in Han Chinese population. Objectives: To develop the rapid and simple Loop-Mediated Isothermal Amplification (LAMP) assay of HLA-B*58:01 and evaluate its feasibility in predicting allopurinol-induced drug hypersensitivity. Methods: Two sets of LAMP primers targeting exons 2 and 3 of HLA B*58:01 were designed. Extracted DNA from twenty clinical blood samples of gout patients were used to evaluate the effectiveness of the two LAMP primers sets for the detection of HLA B*58:01. Results: Results were compared with routine clinical genotyping methods. All extracted DNA samples tested with the HLA-B*58:01 LAMP assay showed agreement with the routine genotyping results. No amplifications were observed when unextracted blood samples were tested. Conclusions: The HLA-B*58:01 LAMP assay was confirmed to be simple, rapid and specific for the detection of HLA-B*58:01 and therefore of potential value in the diagnosis of allopurinol-induced hypersensitivity.
