HUABIO持续追求抗体产品的特异性、稳定性和可重复性，特别推出“华安生物金标抗体”系列产品，从现有的热点领域精选主要靶点与市场竞品进行对比验证。我们的主要目的是基于HiMab重组兔单抗研发核心技术，以BM(Bench Mark)抗体为标准, 为科研工作者提供质量优越，性价比高的优质抗体产品。所筛选与开发出的产品，应用广泛，实验效果理想，且覆盖神经科学、免疫学等多个研究领域：
神经科学领域推荐：
1. BRG1 ET1611-85
2. Vimentin ET1610-39
3. GAP43 ET1610-94
4. Aldolase C HA721733
5. S100 beta ET1610-3
6. Beta III Tubulin M0805-8
7. Ferritin ET1610-78
8. ERK1/2 ET1601-29
9. Erk1 (pT202/pY204) + Erk2 (pT185/pY187) ET1610-13
10. AKT1/2/3 HA721870
11. Ubiquitin ET1609-21
12. AMPK alpha 1 ET1608-40
13. Phospho-GSK3 beta(Ser 9) ET1607-60
fig.1 左侧为华安产品：BRG1 ET1611-85，右侧为对标benchmark
1. 两款产品均ICC 定位均正确，HUABIO (ET1611-85) ICC 应用 可以替代竞品A
2. HUABIO (ET1611-85) 稀释比为 1:1000（2倍于竞品），展示出较高的灵敏性和特异性
Immunocytochemistry analysis of HeLa cells labeling BRG1 with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
fig.2 左侧为华安产品：BRG1 ET1611-85，右侧为对标benchmark
两款产品均能出现单一清晰目标条带，HUABIO (ET1611-85) WB 应用 可以替代benchmark
HUABIO (ET1611-85)稀释比为 1:50,000（5倍于竞品），抗体效价更高
Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: A549 cell lysate (negative)
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
神经科学领域推荐：
1. BRG1 ET1611-85
2. Vimentin ET1610-39
3. GAP43 ET1610-94
4. Aldolase C HA721733
5. S100 beta ET1610-3
6. Beta III Tubulin M0805-8
7. Ferritin ET1610-78
8. ERK1/2 ET1601-29
9. Erk1 (pT202/pY204) + Erk2 (pT185/pY187) ET1610-13
10. AKT1/2/3 HA721870
11. Ubiquitin ET1609-21
12. AMPK alpha 1 ET1608-40
13. Phospho-GSK3 beta(Ser 9) ET1607-60
fig.1 左侧为华安产品：BRG1 ET1611-85，右侧为对标benchmark
1. 两款产品均ICC 定位均正确，HUABIO (ET1611-85) ICC 应用 可以替代竞品A
2. HUABIO (ET1611-85) 稀释比为 1:1000（2倍于竞品），展示出较高的灵敏性和特异性
Immunocytochemistry analysis of HeLa cells labeling BRG1 with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BRG1 antibody (ET1611-85) at 1/1,000 dilution and competitor's antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
fig.2 左侧为华安产品：BRG1 ET1611-85，右侧为对标benchmark
两款产品均能出现单一清晰目标条带，HUABIO (ET1611-85) WB 应用 可以替代benchmark
HUABIO (ET1611-85)稀释比为 1:50,000（5倍于竞品），抗体效价更高
Western blot analysis of BRG1 on different lysates with Rabbit anti-BRG1 antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: A549 cell lysate (negative)
Lane 4: NIH/3T3 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: C6 cell lysate
Lane 7: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 185 kDa
Observed band size: 200 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-85) at 1/50,000 dilution and competitor's antibody at 1/10,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
